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أخواني في الله أحضرت لكم كتاب Make - DIY Science: Illustrated Guide to Home Biology Experiments All Lab, No Lecture Robert Bruce Thompson, Barbara Fritchman Thompson Master the use of the microscope, including sectioning and staining Build and observe microcosms, soda-bottle worlds of pond life Investigate the chemistry of life from simple acids, bases, and buffers to complex carbohydrates, proteins, lipids, enzymes, and DNA Extract, isolate, and observe DNA Explore photosynthesis, osmosis, nitrogen fixation, and other life processes Investigate the cell cycle (mitosis and cytokinesis) Observe populations and ecosystems, and perform air and water pollution tests Investigate genetics and inheritance Do hands-on microbiology, from simple culturing to micro-evolution of bacteria by forced selection Gain hands-on lab experience to prepare for the AP Biology exam
![كتاب Make - DIY Science: Illustrated Guide to Home Biology Experiments E_o_e_11](https://i.servimg.com/u/f61/15/36/74/14/e_o_e_11.jpg) و المحتوى كما يلي :
Table of Contents DIY Science: Illustrated Guide to Home Biology Experiments: All Lab, No Lecture Preface Who this Book is For How this Book is Organized Acknowledgments How to Contact Us Thank You Author Bios 1. Introduction Using this Book with Your Curriculum Planning and Scheduling Work Areas Maintaining a Laboratory Notebook Laboratory Notebook Guidelines Laboratory Notebook Format 2. Equipping a Home Biology Laboratory Microscopes and Accessories Microscope Types Compound Microscope Head Style Illumination Type and Power Source Illumination Methods Nosepiece and Objective Lenses Eyepieces (Oculars) Focuser Mechanical Stage Substage Condenser, Diaphragm, and Filter Holder The Final Decision Stereo Microscope Portable Microscopes and Magnifiers Microscope Accessories Cleaning EquipmentImmersion Oil Photomicrography Equipment Slide Preparation Equipment and Supplies Slides Coverslips Butane Lighter or Alcohol Lamp Forceps and Slide Tongs Mountants Biological Stains Coplin Staining Jar Slide Storage Culturing Equipment and Supplies Autoclave Incubator Culturing Containers Petri Dishes Culture Tubes Culture Flasks Culturing Media Culturing Accessories Histology Equipment and Supplies Microtome Dehydration Baths Embedding Blocks General Laboratory Equipment Major Equipment Spectrophotometer Cellphone Spectrophotometer Ultracentrifuge DNA Processing Equipment and Supplies Prepared Slides Specimens 3. Laboratory Safety Prepare Properly Dress Properly Avoid Laboratory Hazards Don’t Do Stupid Things4. Using a Microscope Equipment and Materials Materials from Kit Materials You Provide Background Procedure I-1-1: A Microscope Tour Controlling and Focusing the Light Focusing the Microscope Determining Field of View and Measuring Specimens Positioning and Observing a Slide Build a Darkfield Apparatus from Pocket Change Review Questions 5. Mounting Specimens Equipment and Materials Materials from Kit Materials You Provide Background Procedure I-2-1: Making Wet Mounts Mounting a Hair Specimen Comparing Temporary Mounting Fluids Procedure I-2-2: Making Smear Mounts Procedure I-2-3: Making Hanging Drop Mounts Procedure I-2-4: Making Sectional Mounts Review Questions 6. Staining Equipment and Materials Materials from Kit Materials You Provide Background Procedure I-3-1: Simple Staining Procedure I-3-2: Gram Staining Review Questions 7. Building and Observing Microcosms Equipment and Materials Materials from Kit Materials You Provide BackgroundProcedure II-1-1: Gathering Materials Field Trip! Procedure II-1-2: Building Aquarium Microcosms Procedure II-1-3: Building Winogradsky Column Microcosms Procedure II-1-4: Observing Winogradsky Column Microcosms Review Questions 8. Observing Succession in Aquarium Microcosms Equipment and Materials Materials from Kit Materials You Provide Background Procedure II-2-1: Observe Succession in Microcosms Review Questions 9. Observing the Effects of Pollution in Microcosms Equipment and Materials Materials from Kit Materials You Provide Background Procedure II-3-1: Build Polluted Microcosms Procedure II-3-2: Observe Succession in Polluted Microcosms Review Questions 10. Acids, Bases, and Buffers Equipment and Materials Materials from Kit Materials You Provide Background Procedure III-1-1: Percentage and Molar Concentrations Making Up 1 M Solutions Procedure III-1-2: Effect of Concentration on pH Populating the Reaction Plate Determining the pH of the Solutions Procedure III-1-3: pH of Household Materials Procedure III-1-4: Buffers Preparing a 0.1 M Acetate Buffer Solution Testing the Buffer Solution Review Questions 11. Carbohydrates and LipidsEquipment and Materials Materials from Kit Materials You Provide Background Carbohydrates (Saccharides) Lipids Procedure III-2-1: Investigating Sugars Procedure III-2-2: Investigating Starches Testing Specimens for the Presence of Starch Testing for Starch at the Cellular Level Procedure III-2-3: Investigating Lipids Solubility of Lipids The Grease-Spot Test for Lipids The Effect of Sudan III Stain on Lipids Observing Lipids at the Cellular Level Review Questions 12. Proteins, Enzymes, and Vitamins Equipment and Materials Materials from Kit Materials You Provide Background Proteins Enzymes Vitamins Procedure III-3-1: Investigating Proteins Preparing Specimens Detecting Proteins with the Biuret Test Testing for the Presence of Cystine Denaturing Proteins with Alcohol, Acids, Bases, and HeavyMetal Ions Procedure III-3-2: Investigating Enzyme Catalysis Prepare a Standard Peroxidase Solution Prepare Modified Peroxidase Solutions Prepare a Neutral Solution of Hydrogen Peroxide Test the Activity of Peroxidase Solutions Procedure III-3-3: Assaying Vitamin C Concentration in Urine Make Up a Standardized Vitamin C SolutionAssay Vitamin C Concentrations Review Questions 13. Coacervates Equipment and Materials Materials from Kit Materials You Provide Background Procedure III-4-1: Prepare and Observe a Coacervate Review Questions 14. Extracting, Isolating, and Visualizing DNA Equipment and Materials Materials from Kit Materials You Provide Background Procedure III-5-1: Extracting and Visualizing DNA Review Questions 15. Build a Gel Electrophoresis Apparatus Equipment and Materials Materials from Kit Materials You Provide Background Procedure III-6-1: Making the Gel Casting Container and Comb Procedure III-6-2: Assemble the Apparatus 16. Simulated DNA Separation by Gel Electrophoresis Equipment and Materials Materials from Kit Materials You Provide Background Procedure III-7-1: Prepare Running Buffer Procedure III-7-2: Prepare and Cast the Gel Procedure III-7-3: Load and Run the Dye Specimens Review Questions 17. Chlorophyll and Photosynthesis Equipment and Materials Materials from Kit Materials You Provide BackgroundProcedure IV-1-1: Observing Carbon Dioxide Uptake Procedure IV-1-2: Determining the Effect of Light Intensity on Photosynthesis Procedure IV-1-3: Chromatography of Plant Pigments and Locating Cellular Energy Reserves Review Questions 18. Investigating Osmosis Equipment and Materials Materials from Kit Materials You Provide Background Procedure IV-2-1: Observing Osmosis in Chicken Eggs Review Questions 19. Investigating Cell Division Equipment and Materials Materials from Kit Materials You Provide Background Procedure IV-3-1: Observing Mitosis Review Questions 20. Sampling Plant Populations in a Community Equipment and Materials Materials from Kit Materials You Provide Background Procedure V-1-1: Choosing and Preparing the Survey Area Procedure V-1-2: Surveying the Plant Community Procedure V-1-3: Performing a Population Count Review Questions 21. Observing the Effect of Rhizobia on Plant Growth Equipment and Materials Materials from Kit Materials You Provide Background Procedure V-2-1: Grow Lima Beans With and Without Rhizobia Endnote Review Questions22. Air Pollution Testing Equipment and Materials Materials from Kit Materials You Provide Background Procedure V-3-1: Building Particle Traps Procedure V-3-2: Positioning and Exposing the Particle Traps Procedure V-3-3: Counting and Identifying Particles Review Questions 23. Soil and Water Pollution Testing Equipment and Materials Materials from Kit Materials You Provide Background Procedure V-4-1: Obtaining Soil and Water Specimens Procedure V-4-2: Testing the Reagents Procedure V-4-3: Making Boron Concentration Comparison Standards Procedure V-4-4: Testing Specimens for Boron Review Questions 24. Exploring Mendelian Genetics Equipment and Materials Materials from Kit Materials You Provide Background Procedure VI-1-1: Testing Subjects for the Ability to Taste PTC Procedure VI-1-2: Charting Inheritance of PTC-Tasting Ability Review Questions 25. Observing Specialized Eukaryotic Cells Equipment and Materials Materials from Kit Materials You Provide Background Procedure VII-1-1: Observing Onion Epidermal and Elodea Leaf Cells Procedure VII-1-2: Comparing and Contrasting Eukaryotic Cells Review Questions 26. Preparing Culturing Media Equipment and MaterialsMaterials from Kit Materials You Provide Background Procedure VII-2-1: Preparing Normal Saline and Nutrient Media Review Questions 27. Culturing Bacteria Equipment and Materials Materials from Kit Materials You Provide Background Procedure VII-3-1: Staining and Observing the Original Culture Procedure VII-3-2: Inoculating Plates and Tubes Procedure VII-3-3: Producing Pure Cultures Review Questions 28. Investigating Bacterial Antibiotic Sensitivity Equipment and Materials Materials from Kit Materials You Provide Background Procedure VII-4-1: Testing Antibiotic Sensitivity Procedure VII-4-2: Culturing an Antibiotic-Resistant Bacteria Strain Procedure VII-4-3: Retesting Sensitivity of the Resistant Strain Review Questions 29. Investigating Protista Equipment and Materials Materials from Kit Materials You Provide Background Procedure VIII-1-1: Observing Spirogyrae Procedure VIII-1-2: Observing Euglenae Procedure VIII-1-3: Observing Amoebae Procedure VIII-1-4: Observing Paramecia Review Questions 30. Investigating Fungi Equipment and Materials Materials from Kit Materials You ProvideBackground Procedure IX-1-1: Observing Zygomycota Procedure IX-1-2: Observing Ascomycota Procedure IX-1-3: Observing Basidiomycota Review Questions 31. Investigating Simple Plants: Mosses and Ferns Equipment and Materials Materials from Kit Materials You Provide Background Procedure X-1-1: Observing Moss Structures Procedure X-1-2: Observing Fern Structures Review Questions 32. Investigating Seed Plants Equipment and Materials Materials from Kit Materials You Provide Background Procedure X-2-1: Observing Germination of a Seed Plant Procedure X-2-2: Observing Root Structures Procedure X-2-3: Observing Stem Structures Procedure X-2-4: Observing Leaf Structures Procedure X-2-5: Observing Reproductive Structures Review Questions 33. Investigating Porifera and Cnidaria Equipment and Materials Materials from Kit Materials You Provide Background Procedure XI-1-1: Observing Porifera Procedure XI-1-2: Observing Cnidaria Review Questions 34. Investigating Platyhelminthes, Nematoda, and Annelida Equipment and Materials Materials from Kit Materials You Provide BackgroundProcedure XI-2-1: Observing Platyhelminthes (Flatworms) Procedure XI-2-2: Observing Nematoda (Roundworms) Procedure XI-2-3: Observing Annelida (Segmented Worms) Review Questions 35. Investigating Arthropods Equipment and Materials Materials from Kit Materials You Provide Background Procedure XI-3-1: Observing and Comparing Arthropod Structures Procedure XI-3-2: Observing Insect Metamorphosis Review Questions 36. Investigating Vertebrate Tissues Equipment and Materials Materials from Kit Materials You Provide Background Procedure XI-4-1: Observing Epithelial Tissues Procedure XI-4-2: Observing Connective Tissues Procedure XI-4-3: Observing Muscle Tissues Procedure XI-4-4: Observing Nervous Tissues Review Questions Index About the Authors
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